Composite

Part:BBa_K1674003

Designed by: Adi Yannai   Group: iGEM15_Technion_Israel   (2015-09-15)

tsPurple Reporter + 3-alpha-HSD (RBS + Coding + Terminator)

 

This part was created especially for checking the expression of the basic part we created - 3αHSD (BBa_K1674001). The 3αHSD stands in the heart of our project – degradation of the dihydrotestosterone (DHT) which is the main cause of male baldness. The part composed of pT7+RBS (BBa_K1321338), 3αHSD and tsPurple Reporter cloned into pSB1C3, which allows the over production of our enzyme in E.coli BL21 cells.

 

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None