Part:BBa_K1670008

Tyrosinase

BBa_K1670008 codes for tyrosinase from Marinomonas mediterranea, a gram negative bacterium, which should facilitate expression in E. coli. Tyrosinases are native to several eukaryotic and prokaryotic species including fungi and bacteria. In its endogenous form, the tyrosinase is a part of the melanin biosynthesis pathway, converting L-Tyrosine to L-DOPA and further to dopaquinone. Melanins, dark pigments, protect the bacterial cells and spores against UV radiation, bind cytotoxic heavy metals, confer protection against oxidants, heat, enzymatic hydrolysis, antimicrobial compounds and phagocytosis (1).

Tyrosinase from M. mediterranea requires the chaperone BBa_K1670009 which is responsible for the delivery of copper to the tyrosinase which enhances production of L-DOPA.(2)

References

1. Claus, Harald ; Decker, Heinz. (2006). Bacterial tyrosinases. Systematic and Applied Microbiology. 29 (1), p3-14.
2. Lopez-Serrano, D., Solano, F. and Sanchez-Amat, A. (2007). Involvement of a novel copper chaperone in tyrosinase activity and melanin synthesis in Marinomonas mediterranea. Microbiology, 153(7), pp.2241-2249.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 588
  Illegal AgeI site found at 616
  Illegal AgeI site found at 1159
  
• 1000
  COMPATIBLE WITH RFC[1000]