Project

Part:BBa_K1662002

Designed by: Jack Kwon   Group: iGEM15_Lambert_GA   (2015-09-04)


Construct 3 Fusion: TetR Promoter:PelB-CDA-eGFP

The purpose of this construct is to produce a single mRNA transcript that it can be translated into one fusion protein. This protein will be led into the periplasm of E. Coli because the PelB leader sequence leads the protein into the periplasm. Th PelB leader sequence leads the protein into the perpiplasm to keep the expression of the CDA gene from killing the E. Coli. The eGFP gene is there to allow the lab workers to see physically (green fluorescent light) that the mRNA transcript has been successfully translated and that the genes are being expressed correctly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 980
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 163
    Illegal AgeI site found at 453
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 564
    Illegal BsaI.rc site found at 1623


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Categories
Parameters
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