Project

Part:BBa_K1662001

Designed by: Janet Standeven Jack Kwon   Group: iGEM15_Lambert_GA   (2015-09-04)

Construct 2 Bidirectional: 2 TetR promoters: PelB-CDA/eGFP

This construct has two TetR promoters going in opposite directions. It also produces two different mRNA transcripts. Like the bicistronic construct, it produces the mRNA transcript with pelB + mature CDA2 and another transcript with just GFP gene. The difference is that this construct only uses one type of promoter while the bicistronic uses two different promoters. The E. coli should glow green which shows that the genes were transcribed and translated correctly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1270
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1135
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 947
    Illegal AgeI site found at 657
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 546
    Illegal BsaI.rc site found at 1913


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