Project

Part:BBa_K1662000

Designed by: Janet Standeven Jack Kwon   Group: iGEM15_Lambert_GA   (2015-08-26)

Construct 1 Bicistronic:PelB-CDA under tetR and GFP under P(Lac)IQ promoter

This construct produces 2 mRNA transcripts. One transcript is under the control of the TetR promoter and it produces the mature CDA2 gene which is led to periplasm by the pelB leader sequence. The other transcript is under the control of the P(Lac) IQ promoter and GFP is produced; E. coli should glow green which means that the genes were successfully transcribed and translated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1081
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 163
    Illegal AgeI site found at 453
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 564
    Illegal BsaI.rc site found at 1724


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Categories
Parameters
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