Composite

Part:BBa_K1658004

Designed by: Şeniz Yüksel Burak Kizil   Group: iGEM15_METU_HS_ANKARA_   (2015-09-15)

Limonene Synthase LS

This is a composite part comprising a constitutive promoter, which is the J23116 promoter f, is itself fused in-frame to limonene synthase from Citrus limon (from part BBa_I742111)and GFP reporter biobrick (E0240). The limonene synthase gene product also carries a C-terminal HA epitope tag. Production of Limonene could have been verified by spectrophotometry. The construct is cloned as an EcoRI / PstI fragment into pSB1C3.

After ligation of I742111 and E0240, we have complete the circuit with constitutive promoter(J23116) and the difference between I742111+E0240 and J231116+I742111+E0240 can be seen by analyzing three lanes after ladder.

Figure1: After ligation of I742111 and E0240, we have complete the circuit with constitutive promoter(J23116) and the difference between I742111+E0240 and J231116+I742111+E0240 can be seen by analyzing three lanes after ladder.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2401
    Illegal SapI.rc site found at 159


[edit]
Categories
//awards/part_collection/2015
Parameters
None