Device

Part:BBa_K1653027

Designed by: Daiki Haraguchi   Group: iGEM15_Nagahama   (2015-09-01)

Geraniol(GOH) production. dev

Geraniol is generated through GPP hydrolysis by geraniol synthase. A MEP pathway has been shown to synthesize IPP and DMAPP efficiently in E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2878
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6665
    Illegal BamHI site found at 5993
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1462
    Illegal AgeI site found at 2174
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3867



E. coli engineered with geraniol production device (BBa_K1653027) showed different smell as compared with counterpart control (pSB1C3) and WT. This result derived from Questionnaire survey. And then we tried to detect that the geraniol generated by engineered "E. coli" by GC and GC-MS. However, the Geraniol were not detected. These result may indicate that E. coli with Geraniol production device produce smaller amouts of than can be detected by GC and GC-MS.
[[http://2015.igem.org/Team:Nagahama/Medal_Parts#Geraniol_.28GOH.29_production_device.28BBa_K1653027.29 here] is our result page.]

fig8：Questionnaire survey of fragrance of geraniol A：WT or recombinant（JM109/empty vector） B：recombinant（JM109/GES） Experimental cooperation persons：20 persons Experiment smelling the smell of A and B Experiment collaborators chose a stronger smell by comparing the A and B.


Fig.10 Result of questionnaire survey using WT and recombinant (JM109/GES). Out of 20 persons, two persons (10%) answered the medium A (WT) smelled stronger than the medium B (recombinant (JM109/GES)) and eighteen persons (90%) answered the medium B smelled stronger than the medium A. If we assume that both media smell equally, the probability that the medium A is selected in the questionnaire must be 0.5. From this assumption, p-value of this result was calculated using binomial test. Because the p-value was much smaller than the 5% significance level (0.0004025), the smell of recombinant (JM109/GES) is stronger than that of WT significantly. This result indicate that the recombinant (JM109/GES) synthesize geraniol.
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