Composite

Part:BBa_K1651001

Designed by: YANG YUANZHAN   Group: iGEM15_BIT   (2015-09-13)

J23100+LasR+LasR/PAI1 Inducible Promoter+RhlI

PAI1(3-oxo-C12-HSL) in -> PAI2(C4-HSL) out

PAI1 can combine with LasR and then activate the LasR & PAI1 regulated promoter. The transcription of RhlI will be activated, which catalyze the production of PAI2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 987
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1773
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None