Composite

Part:BBa_K1651000

Designed by: YANG YUANZHAN   Group: iGEM15_BIT   (2015-09-13)

PAI1 detector

When the PAI1(3-oxo-C12-HSL) spread into the amplifier, it will combine with LasR which is regulated by a constitutive promoter. Then the LasR & PAI regulated promoter will be activated to start the production of RhlI. The RhlI will catalyze the production of PAI2(C4-HSL), which will combine with RhlR to activate the RhlR & PAI2 regulated promoter. Then the production of sfGFP is activated.

The detection limit of this detector is very low(0.005 nmol/L, see more data in experience).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 987
    Illegal NheI site found at 2604
    Illegal NheI site found at 2627
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1773
    Illegal BamHI site found at 2901
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3376
    Illegal BsaI.rc site found at 2513


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Parameters
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