Device

Part:BBa_K165091

Designed by: John Szymanski   Group: iGEM08_BrownTwo   (2008-10-29)


Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS306

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 453
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 453
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2240
    Illegal XhoI site found at 1
    Illegal XhoI site found at 41
    Illegal XhoI site found at 203
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 453
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 453
    Illegal AgeI site found at 3383
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1411
    Illegal SapI.rc site found at 2004


[edit]
Categories
//chassis/eukaryote/yeast
//function/regulation/transcriptional
Parameters
None