Generator

Part:BBa_K165054

Designed by: John Szymanski   Group: iGEM08_BrownTwo   (2008-10-29)


Full Gli1 Repressor (tagged with CFPx2)

Kozak+Sin3p RD+Gli1 DBD+CFPx2+SV40NLS+tADH1


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3541
    Illegal AgeI site found at 4291
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 946
    Illegal SapI.rc site found at 1539


[edit]
Categories
//chassis/eukaryote/yeast
Parameters
None