Regulatory
Part:BBa_K165035
Designed by: John Szymanski Group: iGEM08_BrownTwo (2008-10-29)
Gli1 bs + Zif268-HIV bs + mCYC promoter
The Gli1 and Zif268-HIV transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Gli1 and Zif268-HIV binding transcription factors.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1
Illegal XhoI site found at 122
Illegal XhoI site found at 136
Illegal XhoI site found at 176
Illegal XhoI site found at 188 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 119
[edit]
Categories
Parameters
//rnap/eukaryote/yeast
//direction/forward
//chassis/eukaryote/yeast
//promoter
//regulation/unknown
//direction/forward
//chassis/eukaryote/yeast
//promoter
//regulation/unknown
negative_regulators | -1 |
positive_regulators | -1 |