Regulatory

Part:BBa_K165032

Designed by: Aaron Glieberman   Group: iGEM08_BrownTwo   (2008-10-28)

mCYC promoter plus Gli1 binding sites

This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Gli1 binding domain. This part contains the most of the sequences for BBa_K165012 and BBa_K165016 but also has some additional DNA and a few differences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
    Illegal XhoI site found at 145
    Illegal XhoI site found at 157
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 142


[edit]
Categories
//rnap/eukaryote/yeast
//direction/forward
//chassis/eukaryote/yeast
//promoter
//regulation/unknown
Parameters
negative_regulators-1
positive_regulators-1