Intermediate

Part:BBa_K165025

Designed by: John Szymanski   Group: iGEM08_BrownTwo   (2008-10-27)


Kozak + Sin3 repression domain + LexA DNA-binding domain

This is the kozak sequence and Sin3 transcriptional repression domain ligated to the LexA DNA-binding domain. It can be used to build synthetic transcription repressors that bind to the lex operator by ligating it to (FPx2*)+(NLS+term)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 946
    Illegal SapI.rc site found at 1539


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Categories
Parameters
None