Intermediate

Part:BBa_K165019

Designed by: John Szymanski   Group: iGEM08_BrownTwo   (2008-10-27)


CFPx2 + SV40 NLS + ADH1 yeast terminator

This intermediate consists of two copies of CFP fused to the SV40 nuclear localization sequence, ending with the ADH1 terminator. It can be used to build synthetic transcription factors for use in yeast by ligating it to a construct containing Kozak+(repression/activation) domain + DNA-binding domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 163
    Illegal AgeI site found at 913
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None