Coding
CDI

Part:BBa_K1650013

Designed by: Daniel Stukenberg   Group: iGEM15_Marburg   (2015-09-18)

This part contains the CDI operon

This part contains The Cdi system organized in an operon consisting of three genes:

  • CdiB is a pore forming protein with a β-barrel structure and might be needed in secretion and assembly of CdiA (Aoki et al., 2011).
  • CdiA is the toxin delivering protein. The C-terminus (CT) of CdiA acts as toxic domain and got various orphan CdiA-CT units in behind (Poole et al., 2011).
  • The third gene is CdiI, the inhibitor to protect the cells against their own toxins.


The image below shows the basic principle of the CDI system. The CdiA protein of CDI+ cells get in contact with the BamA receptor located in the outer membrane leading to proteolytic cleavage of the inhibitory C-terminal domain. This C-terminal domain acts as growth inhibiting factor if the target cell does not carry the CdiI inhibitor. If the CdiI inhibitor is present the toxin is unfunctional. 


 MR-pic_CDI_cartoon.JPG

This part can be used to grant your strain the ability to inhibit the growth of gram negativ bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5910
    Illegal PstI site found at 356
    Illegal PstI site found at 541
    Illegal PstI site found at 1036
    Illegal PstI site found at 1111
    Illegal PstI site found at 2143
    Illegal PstI site found at 2290
    Illegal PstI site found at 2429
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5910
    Illegal PstI site found at 356
    Illegal PstI site found at 541
    Illegal PstI site found at 1036
    Illegal PstI site found at 1111
    Illegal PstI site found at 2143
    Illegal PstI site found at 2290
    Illegal PstI site found at 2429
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5910
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5910
    Illegal PstI site found at 356
    Illegal PstI site found at 541
    Illegal PstI site found at 1036
    Illegal PstI site found at 1111
    Illegal PstI site found at 2143
    Illegal PstI site found at 2290
    Illegal PstI site found at 2429
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5910
    Illegal PstI site found at 356
    Illegal PstI site found at 541
    Illegal PstI site found at 1036
    Illegal PstI site found at 1111
    Illegal PstI site found at 2143
    Illegal PstI site found at 2290
    Illegal PstI site found at 2429
    Illegal NgoMIV site found at 2164
    Illegal NgoMIV site found at 2659
    Illegal NgoMIV site found at 4234
    Illegal NgoMIV site found at 5788
    Illegal AgeI site found at 799
    Illegal AgeI site found at 1973
    Illegal AgeI site found at 2026
    Illegal AgeI site found at 3061
    Illegal AgeI site found at 3826
    Illegal AgeI site found at 4117
    Illegal AgeI site found at 4141
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1523
    Illegal BsaI site found at 6354
    Illegal BsaI site found at 6775
    Illegal BsaI.rc site found at 1031
    Illegal BsaI.rc site found at 1298
    Illegal BsaI.rc site found at 10036


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