Plasmid

Part:BBa_K1650011

Designed by: Matthias Franz   Group: iGEM15_Marburg   (2015-09-18)

sRNA Anti-MinC leading to Minicell Production

This plasmid encodes a sRNA with 24 bp which is complementary to a sequence in the mRNA of MinC thus, leading to a prevention of its biosynthesis. Since the Min-system regulates symmetric cell division, this sRNA stimulates the formation of minicells which do not contain chromosomal DNA and therefore, are not able to propagate making them a chassis with a low risk in biosafety. The plasmid contains additionally a high-copy origin of replication and a cassette for the resistance against Chloramphenicol.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 293
    Illegal EcoRI site found at 2341
    Illegal XbaI site found at 2356
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2341
    Illegal SpeI site found at 294
    Illegal PstI site found at 308
    Illegal NotI site found at 301
    Illegal NotI site found at 2347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2341
    Illegal XhoI site found at 153
    Illegal XhoI site found at 159
    Illegal XhoI site found at 1325
    Illegal XhoI site found at 2217
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2341
    Illegal suffix found in sequence at 294
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2341
    Illegal XbaI site found at 2356
    Illegal SpeI site found at 294
    Illegal PstI site found at 308
  • 1000
    COMPATIBLE WITH RFC[1000]


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