Composite
Part:BBa_K1649004
Designed by: Yang Yang Group: iGEM15_NJAU_China (2015-09-07)
YF1-FixJ with terminator
The LOV photo-switch YF1 was created by fusing the light perception domain of YtvA to the FixL kinase domain. YtvA acts as a blue-light photoreceptor and carries a blue-light-sensitive flavin-binding LOV domain. The response regulator FixJ forms a two-component system together with FixL.The sensor is inactive in light and active in the dark.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 562
Illegal NgoMIV site found at 634
Illegal NgoMIV site found at 724
Illegal NgoMIV site found at 742
Illegal NgoMIV site found at 1254
Illegal NgoMIV site found at 1547
Illegal NgoMIV site found at 1641
Illegal AgeI site found at 276
Illegal AgeI site found at 1422 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1311
Illegal BsaI.rc site found at 175
[edit]
Categories
Parameters
None |