Composite
RBS oprF

Part:BBa_K1648047

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-05-31)

AvOprF with RBS

Construct Map for K1648047

OprF is a transmembrane porin which allows non-specific transmission. For constructing an efficient microbial fuel cell(MFC), we manipulated the expression of oprF. oprF allows electron carriers shuttle in and out of the bacteria, the electron generator. The electrons generated by the bacteria are taken up by the incoming carrier. Reduced electron carriers transport the electrons out of the bacteria to the electrode. BBa_K1648046 is the intermediate part which involves addition of RBS in BBa_K1648045.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 493


[edit]
Categories
Parameters
n/aAvOprF with RBS