Part:BBa_K1647014

Lac promoter+CI+B0010

This part contains an IPTG induced promoter and a CI repressor. Isopropyl β-D-1-thiogalactopyranoside (IPTG) is a biological reagent which is a molecular mimic of allolactose and triggers trancrsiption of the lac operon and subsequent gene expression. The lac repressor, LacI, is also caused to dissociate from the promoter gene when lactose or allolactose-like module is present. LacI also binds to the major groove in the operator sequence and blocks T7 RNA polymerase from binding the promoter sequence. IPTG is therefore required in order to enable the binding and action of T7 RNA polymerase to transcribe the plasmid, or DNA fragment with T7 promoter. The IPTG promoter used in this system was Part BBa_R0010.This part is under the control of IPTG, when IPTG exists, CI repressor can be expressed.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 894
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]