Intermediate

Part:BBa_K1647013

Designed by: Yun Xue   Group: iGEM15_WHU-China   (2015-09-15)

J23100+taRNA

This part is J23100+taRNA+terminator.The promoter J23100 is a strong promoter, and it comes from the constitutive promoter family, these promoter parts can be used to tune the expression level of constitutively expressed parts. The NheI and AvrII restriction sites present within these promoter parts make them a scaffold for further modification. taRNA is a short sequence which can act as the "key" to riboregulator when transcripted into mRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 41


[edit]
Categories
Parameters
None