Composite

Part:BBa_K1647012

Designed by: Zhixiong Xie   Group: iGEM15_WHU-China   (2015-09-15)

PompC+taRNA+B0010

This part is to generate taRNA which can unlock the CrRNA by competing with the RBS at the absence of illumination. In nature, this promoter is upstream of the ompC porin gene. The regulation of ompC is determined by the EnvZ-OmpR osmosensing machinery. EnvZ phosphorylates OmpR to OmpR-P. At high osmolarity, EnvZ is more active, creating more OmpR-P. OmpR-P then binds to the low-affinity OmpR operator sites upstream of ompC. When red light exists, PompC is activated, and taRNA is produced, taRNA is a short sequence which can act as the "key" to riboregulator when transcripted into mRNA, so it can pair with a spectacular riboregulator, and unlock it, making it non-functional. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 114


[edit]
Categories
Parameters
None