Intermediate

Part:BBa_K1647010

Designed by: Zhixiong Xie   Group: iGEM15_WHU-China   (2015-09-15)

CI+B0010

This part is composed of CI repressor and a terminator. The CI repressor is from E. coli phage lambda, and it is followed by a LVA tag. Coding region for the cI repressor based on CI repressor from bacteriophage lambda modified with an LVA tag for rapid degradation of the protein. CI repressor binds to the CI regulator (BBa_R0051). An alternate version of this part is available without a barcode: BBa_K327018.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None