Composite

Part:BBa_K1647009

Designed by: Zhixiong Xie   Group: iGEM15_WHU-China   (2015-09-15)

PompC+Riboregulator+CI+B0010+PompC+taRNA+B0010

This part can be divided into two functional parts: PompC+riboregulator+RBS+CI+Terminator, and PompC+taRNA+Terminator. The former part function as follows: when the red light exists, the ompC promoter is activated, and the CI repressing protein’s expression is depressed, so the expression of CI repressor is controlled by the state of red light. The later part works as the key of the former part, when red light exists, the promoter PompC is activated, and taRNA is expressed, then this taRNA can bind to the riboregulator and unlock it, so the CI repressor can be expressed again.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 850
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1066


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Categories
Parameters
None