Part:BBa_K1647006
J23100+B0034+Cph8+B0034+ho1+B0034+pcyA+B0010
A functional red light sensor. It activates the ompC promoter when red light intensity is low while inhibits when red light intensity is higher than a certain threshold. The light-sensing protein we use is Cph8, a chimeric protein which produces strong response to light. It consists of a light-sensing domain Cph1 and an EnvZ domain. Phococyanobilin is necessary for Cph1 to response to light, but it's not naturally produced in E Coli. Another two genes, ho1 and pcyA, can produce two enzymes that convert the endogenous haem to phococyanobilin. Functioning as a kinase, the EnvZ domain could lead the autophosphorylation of an endogenous regulator OmpR, which activates the ompC promoter, when light intensity keeps below a certain threshold. But when light intensity is high, autophosphorylation is inhibited and therefore, the expression stops.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3870
Illegal XhoI site found at 427 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3423
- 1000COMPATIBLE WITH RFC[1000]
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