Composite

Part:BBa_K1647005

Designed by: Yun Xue   Group: iGEM15_WHU-China   (2015-09-15)

J23100+Cph8

Cph8 is a chimeric protein which can response to light. It consist of a light-sensing domain Cph1 and an EnvZ domain. Phococyanobilin is necessary for Cph1 to response to light, but it's not naturally produced in E Coli. Therefore, another two genes, ho1 and pcyA, must be introduced. Functioning as a kinase, the EnvZ domain could lead the autophosphorylation of an endogenous regulator OmpR, which activates the ompC promoter, when light intensity keeps below a certain threshold. But when light intensity is high, autophosphorylation is inhibited and therefore, the expression stops.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 427
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None