Intermediate

Part:BBa_K1647004

Designed by: Yun Xue   Group: iGEM15_WHU-China   (2015-09-15)

B0034+ho1+B0034+pcyA

A set of enzymes that could synthesize phycocyanobilin in E. Coli under the control of a strong RBS B0034. Ho1 is one member of the heme oxygenases family. It functions in producing BV IXa from endogenous heme in E. Coli. Ho1 catalyses stereospecific cleavage of heme and releases Fe2+ and carbon monoxide, which is the first step of phycocyanobilin synthesis. The second step is conducted by a phycocyanobilin:ferredoxin oxidoreductase (pcyA) which functions in reducing BV IXa.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1564
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1117
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None