Coding

Part:BBa_K1641901

Designed by: Chunyang Ni   Group: iGEM15_SYSU_CHINA   (2015-09-12)


bxb1gp35 optimized for RFC25 with SV40NLS

add SV40NLS via PCR

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 192
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 466
    Illegal XhoI site found at 553
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1300


[edit]
Categories
Parameters
None