Coding

Part:BBa_K1641900

Designed by: Chunyang Ni   Group: iGEM15_SYSU_CHINA   (2015-09-10)

bxb1gp35 optimized for RFC25

bxb1gp35 is a ser reombinase, which catalyzes site-specific recombination between Bxb1 attP and attB sites. When attP and attP sites are oppositely located, recombinase bxb1 can mediate an inversion betwen attB/P sites, forming attL/R sites.

The bxb1 integrase part has been submitted by iGEM2013-Waterloo (BBa_K1039003), but it does not match the RFC25 standard and thus can not be applied on fusion via 3A assembly.

In SYSU-CHINA 2015, we modified the bxb1 gene to make it match the RFC25 standard, using the optimized bxb1gp35 to catalyze the inversion of segments flanked by attB/P sites, creating a counter(Micro timer 2.0).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 192
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 466
    Illegal XhoI site found at 553
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1300


[edit]
Categories
Parameters
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