DNA

Part:BBa_K1641220

Designed by: Jianheng Liu   Group: iGEM15_SYSU_CHINA   (2015-09-14)


pBAD(forward)-FRT(forward)-loxP(reverse)

This sequence has two primer sites and a restriction enzyme site between FRT and loxP for further testing in our experiment. Two primers are: GCACCTTGACTCTGACAATCCT (forward) GCCTGTGCTAATGGTGATGACT (reverse) they both have a high annealing temperature (about 58C). They can be used as qPCR primers. The restriction enzyme sites are HindIII and BanI.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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