DNA

Part:BBa_K1641201

Designed by: Jianheng Liu   Group: iGEM15_SYSU_CHINA   (2015-09-14)


pBAD(forward)-FRT(forward)-loxP(reverse)-pBAD(reverse)

Constructed intermediate for prokaryotic system in iGEM15_SYSU_CHINA which did not shipped to iGEM HQ. Pleased note that the opposite pBADs will form a secondary structure during transcribing and sequencing, but we still detect ECFP signal after induction. Two primers in part BBa_B1641220 will help sequencing and testing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 295
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 355
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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