Translational_Unit

Part:BBa_K1641018

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-10)


Up-side-down RBS::mcherry-ssra with SloxM1, Scre recognition sites

This is a special RBS::mcherry-ssra inverted into up-side-down form, with two SloxM1 sites at the upstream and downstream of the gene. SloxM1 can be recognized by invertase Scre and the middle mcherry can be restored through inversion. Ssra-tag in this sequence is moderately fast type (BBa_M0052) that accelerates the degradation but at a speed far less than expression.

This part can be used to construct the reporter for real-time invertase dynamics analysis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None