Translational_Unit

Part:BBa_K1641016

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-10)


Up-side-down RBS::mcherry-ssra with Rox, recognition site of Dre

This is a special RBS::mcherry-ssra inverted into up-side-down form, with two Rox sites at the upstream and downstream of the gene. Rox can be recognized and the middle sequence can be restored by invertase Dre (BBa_K1641002). Ssra-tag in this sequence is moderately fast type (BBa_M0052) that accelerates the degradation but at a speed far less than expression.

This part can be used to construct the reporter for real-time invertase dynamics analysis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None