DNA

Part:BBa_K1641013

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-10)


SloxM1, modified recognition site of recombinase Scre

This is a recognition site of Cre-like recombinase Scre, that is "CTCGTGTCCGATA ACTGTAAT TATCGGACACGAG". The original recognition site of Scre is SloxP, "CTCGTGTCCGATA ACTGTAAT TATCGGACATGAT", contains ATG that might be used as Met to start translation. So two mutation is conducted to avoid ATG and although this generates slight mismatch, this will not significantly reduce the activity of Scre on this site.

This recognition site is not homologous with LoxP and hence will not cross-react with Cre or other common recombinases.

Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. The scar will be "ACTAGA" safely without cutting activity and start code. with This deletion do not interfere the usage of the brick.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None