Part:BBa_K1641007

Fusion protein of Scre::EGFP::ssra-tag, with RBS at beginning

This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Scre::EGFP::ssra-tag. The part is for expression of Scre::EGFP::ssra-tag, which aims to overturn our invertase modules in our Micro-timer.

Scre (BBa_K1641001) in our bricks is a new-discovered Cre-like recombinase with invertase activity that turns the sequence between two anti-parallel SloxP (or its derivatives) sites up-side-down. An EGFP fusion at the C-term of Scre will not significantly interfere the enzymatic activity, and a flexible chain of 8 AA is added between the two parts to further avoid side-effect like misfolding. An ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need and reduce the leakage expression.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]