Translational_Unit

Part:BBa_K1641006

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-09)


Fusion protein of Vcre::EGFP::ssra-tag, with RBS at beginning

This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Vcre::EGFP::ssra-tag. The part is for expression of Vcre::EGFP::ssra-tag, which aims to overturn our invertase modules in our Micro-timer.

Vcre (BBa_1641000) in our bricks is a new-discovered Cre-like recombinase with invertase activity that turns the sequence between two anti-parallel VloxP sites up-side-down. A EGFP fusion at the C-term of Vcre will not significantly interfere the enzymatic activity. A ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need and reduce the leakage expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None