Translational_Unit
Part:BBa_K1641004
Designed by: Pai Li Group: iGEM15_SYSU_CHINA (2015-09-09)
Fusion protein of Cre::EGFP::ssra-tag, with RBS at beginning
This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Cre::EGFP::ssra-tag.
Cre is a most commonly used recombinase with invertase activity that turns the sequence between two anti-parallel LoxP sites up-side-down. A EGFP fusion at the C-term of Cre has been proved by us not to significantly interfere the enzymatic activity. A ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need.
This part is for expression of Cre::EGFP::ssra-tag, which aims to overturn our invertase modules in our Micro-timer.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 286
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |