Coding

Part:BBa_K1641002

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-08)

Recombinase Dre, E. coli and yeast coding optimized

This is a Cre-like recombinase of Tyr-family with properties similar to those of Cre, but with a different recognized sequence Rox (or its proper derivatives), which do not cross-interfering with Cre-LoxP system. Dre is one of the most widely used Cre-like recombinase.

Dre has the invertase activity that invert the the sequence between two anti-parallel Roxs. Dre is a integrase used by P1-like coliphage, yet this coding sequence is not the wild type sequence but redesigned into E. coli-and-yeast coding preference according to its AA sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None