Part:BBa_K1641001
Recombinase Scre, E. coli and yeast coding optimized
This is a Cre-like recombinase of Tyr-family with properties similar to those of Cre, but with a different recognized sequence SloxP (or its proper derivatives), which do not cross-interfering with Cre-LoxP system. However, because the wild type SloxP contains consecutive ATG that takes risk to unexpectedly initiate protein translation, we highly recommend to use an derivative of SloxP, SloxM1 as the target of Scre.
Scre has the invertase activity that invert the the sequence between two anti-parallel SloxM1. The original Scre is discovered through genome screening of Shewanella sp. ANA-3, yet this coding sequence is redesigned into E. coli-and-yeast coding preference according to its AA sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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