Coding

Part:BBa_K1641000

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-08)


Recombinase Vcre, E. coli-and-yeast coding optimized

This is a Cre-like recombinase of Tyr-family with properties similar to those of Cre, but with a different recognized sequence VloxP, which do not cross-interfering with Cre-LoxP system. Vcre has the invertase activity that invert the the sequence between two anti-parallel Vloxp. The original Vcre is discovered through genome screening of Vibrio sp. 0908, yet this coding sequence is redesigned into E. coli-and-yeast coding preference from original protein sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None