Part:BBa_K1640022

psbP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 537
  
• 1000
  COMPATIBLE WITH RFC[1000]

Overview

This biobrick contains a gene from the photosystem II complex of Chlamydomonas reinhardtii - psbP, with RBS included.

This part was designed in conjunction with BBa_K1640012 and BBa_K1640023, together they form operon 5 in our set of photosystem II operons:

Biology & Literature

The psbP gene that comprises this part transcribes a protein that optimizes the availability of Ca2+ and Cl- cofactors in the Oxygen Evolving Complex in PSII to maintain the active Manganese cluster.

Protein information

psbP

mass: 23.17kDa

sequence:
MASGSDVSRRAALAGFAGAAALVSSSPANAAYGDSANVFGKVTNKSGFVPYAGDGFALLLPAKWNPSKENDF PGVILRYEDNFDAVNNLVVIAQDTDKKAIADFGSQDKFLESVSYLLGKQAYSGETQSEGGFAPNRVSAASLL DVSTTTDKKGKTYYKYELLVRSADGDEGGRHQLIGATVGSDNKLYIIKIQIGDKRWFKGAKKEAMGAFDSFT VV*

Part verification

Visualisation of psbP showing expected banding is shown in the following gel image, bottom row third set of lanes, with left lane part showing EcoRI digest, right lane showing EcoRI + PstI double digest. This part has been sequenced to confirm design with final biobrick.