Regulatory
pLac

Part:BBa_K1638000

Designed by: Jens Sivkśr Pettersen   Group: iGEM15_SDU-Denmark   (2015-05-07)

Lac operator

A regulator sensitive to the Lac Repressor (encoded by the regulator gene LacI) and the catabolite activator protein (CAP).

The regulator contains two binding sites. The first is bound by the CAP protein, which is generally present in E. coli and is asocciated with cell health and availbaility of glucose. CAP contains a helix-turn-helix motif and binds major-groove DNA in the presence of the allosteric effector cyclic-AMP at the following site: 5'-AATGTGAGTTAGCTCACTCATT-3'. The second site, 5'-AATTGTGAGCGGATAACAA-3', is bound by the LacI repressor protein. When ispopropyl beta-D-1-thiogalactopyranoside (IPTG), which is an analog to allolactose, binds, LacI is inhibited.

In the absence of LacI protein and CAP protein, this part promotes a basal level of transcription. In the presence of LacI protein and CAP protein, this part inhibits transcription. In the absence of LacI protein and the presence of CAP protein, this part promotes a high level of transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None