Primer

Part:BBa_K1636001

Designed by: Roger Milton Rubio Sanchez   Group: iGEM15_TecCEM   (2015-09-05)

Primer for lead-II-ions-specific aptamer

This primer was designed specifically to amplify the lead II aptamer sequence within a biobrick (flanked by prefix and suffix) and obtain it in a single-strand fashion. Thus, this part is intimately related to BBa_K1636000

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
directionF
pct_gc60%
targetBBa_K1636000
t_m40