Regulatory

Part:BBa_K1634002

Designed by: Xinhong Chen   Group: iGEM15_China_Tongji   (2015-09-13)

pmyo2 ( a promoter in C.elegans )

Pmyo2 is a promoter which can drive the expression in the muscle of C.elegans especially the muscle in the head and pharynx. And we link the Pmyo2 with some channelrhodopsin which can be activated or suppressed by the light. Since we have learned that the choosing of direction in C.elegans depends on the muscle in the head, we can observe the obvious change in moving pattern of the C.elegans after we shed the light. In other words, we can use them to construct a light-sensed locomotion controlling system in C.elegans. And we achieve that goal by using this part to control the movement of the head, thus controlling the movement of the whole C.elegans.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 288
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 198


[edit]
Categories
//regulation
Parameters
None