Part:BBa_K1632031
fim switch[default OFF](Tokyo_Tech/J23119)_rbs_gfp_rbs_rhlI
The fim switch is the promoter containing repeated DNA sequence which is inverted by the Fim recombinase. Therefore, we can control the expression of the gene downstream of the fim switch by adding the Fim recombinase.
We designed this fim switch which has a J23119 promoter. Also between the promoter and the inverting site, there are two restriction enzyme sites in each front (SalIand BamHI) and back (BglII and MluI)(Fig. 1. Tokyo Tech fim switch (Tokyo_Tech/J23119) design). So the promoter can easily be interchanged. Except for insertion of restriction enzyme sites, basically, the design of TokyoTech fim switch is similar with Wild Type fim switch.
When the state of fim switch is ON, GFP and C4HSL are expressed. But when the state of fim switch is off, GFP and C4HSL are not expressed.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 98
Illegal NheI site found at 121 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 92
Illegal BglII site found at 1865
Illegal BamHI site found at 133 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1102
| None |