Colicin-E3 cassette (Medium)
Colicin-E3, its immunity protein, and colisin lysis protein is constitutively expressed.(under BBa_J23110) When this device works, Colicin-E3 is released to media, and kill E. coli strains which does not have immunity protein.
Usage and Biology
Colicins are a cytotoxins which are released to environment and kill other related strains.
Release of colicin involves one protein; Colicin Lysis Protein. Colicin lysis protein allows colicins to be released. The mechanism how the colicin lysis protein allows colicin release has not been fully elucidated, but it is sure that this protein raise membrane permitability and cause quasilysis. After Colicin released, they diffuse through the medium and bind to the receptor on the target cell membrane. Then, they are imported to the cytoplasm or cytoplasmic membrane of target cell by Tol-system or Ton-system. (If you want to know the mechanism of the Colicin import, see ) Colicin producing cells also express Colicin Immunity Protein in order to protect themselves from cytotoxity of colicin. Colicins have variety cytotoxity such as DNase activity, RNase activity or Pore forming across inner membrane.
Reference Cascales, E., Buchanan, S. K., Duché, D., Kleanthous, C., Lloubes, R., Postle, K., ... & Cavard, D. (2007). Colicin biology. Microbiology and Molecular Biology Reviews, 71(1), 158-229.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21Illegal BglII site found at 1733
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 696
Illegal AgeI site found at 68
Illegal AgeI site found at 179
Illegal AgeI site found at 448
Illegal AgeI site found at 1714
Illegal AgeI site found at 1819
- 1000COMPATIBLE WITH RFC