Generator

Part:BBa_K1631014

Designed by: Yuto Yamanaka   Group: iGEM15_UT-Tokyo   (2015-09-14)


Pconst(strong) -rbs- Colicin E3 -rbs- Colicin Immunity protein -d.tem

Colicin-E3 and its immunity protein is co-expressed under BBa_J23101

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1569
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 532
    Illegal AgeI site found at 284
    Illegal AgeI site found at 1550
    Illegal AgeI site found at 1655
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None