Plasmid

Part:BBa_K163101

Designed by: Daniel M. Choi   Group: iGEM08_University_of_Chicago   (2008-10-11)


pUC8 Plasmid DNA

pUC8 is a plasmid that has the last 336 AA of RsaA (Caulobacter surface protein) and it allows for a cloning of desired segment into a EcoR1, Sma1 and or BamH1 site upstream of the 336.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2016
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2016
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2016
    Illegal BamHI site found at 2026
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2016
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2016
    Illegal NgoMIV site found at 2863
    Illegal NgoMIV site found at 3143
    Illegal AgeI site found at 2203
    Illegal AgeI site found at 2575
    Illegal AgeI site found at 2986
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3088
    Illegal SapI.rc site found at 1782


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