Composite

Part:BBa_K1631008

Designed by: Yuto Yamanaka   Group: iGEM15_UT-Tokyo   (2015-09-14)


rbs - ColicinE3 - rbs - Colicin Immunity protein - d.term

When this is placed under a promoter, Colicin-E3(BBa_K1631002) is co-expressed with its immunity protein(BBa_K1631004).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1526
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 489
    Illegal AgeI site found at 241
    Illegal AgeI site found at 1507
    Illegal AgeI site found at 1612
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None