Coding

Part:BBa_K163002

Designed by: Nora Yucel   Group: iGEM08_University_of_Chicago   (2008-08-25)


Mussel adhesive protein from Mytilus Edulis (Mefp-5) optimized for E. coli

This is the protein Mefp-5 that is secreted by Mytilus Edulis. Mefp-5 is one of the strongest adhesives naturally created, and a powerful anti-biofouling agent. The DNA has been codon optimized for use in E. coli using open-software codon-optimizer at http://genomes.urv.es/OPTIMIZER/. This gene lacks a promoter sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 318
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 318
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 338
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 318
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal SpeI site found at 318
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None