Coding
Part:BBa_K163002
Designed by: Nora Yucel Group: iGEM08_University_of_Chicago (2008-08-25)
Mussel adhesive protein from Mytilus Edulis (Mefp-5) optimized for E. coli
This is the protein Mefp-5 that is secreted by Mytilus Edulis. Mefp-5 is one of the strongest adhesives naturally created, and a powerful anti-biofouling agent. The DNA has been codon optimized for use in E. coli using open-software codon-optimizer at http://genomes.urv.es/OPTIMIZER/. This gene lacks a promoter sequence.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal SpeI site found at 318 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 318
Illegal NotI site found at 7 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal BglII site found at 338 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal SpeI site found at 318 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal SpeI site found at 318 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |